標(biāo)記核苷酸是生命科學(xué)研究的關(guān)鍵要素,是分子生物學(xué)中檢測(cè)特定核苷酸序列的有效工具,通過(guò)酶的作用即可結(jié)合到DNA/RNA序列中,進(jìn)行測(cè)序和分析。由于出色的安全性、穩(wěn)定性和便捷性,生物素和熒光標(biāo)記是用于原位雜交、基因表達(dá)譜分析或電泳遷移率轉(zhuǎn)移分析(EMSA)的核酸探針的最常見(jiàn)標(biāo)記。
產(chǎn)品應(yīng)用
核酸序列測(cè)定(Southern/Northern印記)
Hi-C技術(shù),捕捉染色質(zhì)構(gòu)象
菌落與噬菌體,細(xì)胞與染色體的原位雜交
基因表達(dá)分析
治病病原體檢測(cè)
遺傳病與腫瘤診斷
品名:Aminoallyl-dUTP-XX-ATTO-532 Rhodamine-12-dUTP
貨號(hào):NU-803-RHOX
Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
希凱生物是德國(guó)Jena Bioscience授權(quán)代理商,更多產(chǎn)品信息歡迎直接與我們聯(lián)系